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. 2012 Jun 15;24(6):2528–2545. doi: 10.1105/tpc.112.098475

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© 2012 American Society of Plant Biologists. All rights reserved.

PMC Copyright notice

Figure 2. — Rescue Assays in L. japonicus Mutants with Lj-CASTOR, Lj-POLLUX, DMI1, and Modified Alleles.

(A) to (C) Rescue of the L. japonicus castor pollux double mutant by Lj-CASTOR, Lj-POLLUX, and DMI1.

(A) Lj-CASTOR rescued only castor but failed to rescue the Lj-castor pollux double mutant.

(B) Lj-POLLUX rescued only Lj-pollux but failed to rescue the Lj-castor pollux double mutant.

(C) DMI1 rescues both single and double mutant Lj-castor pollux.

(D) to (F) Rescue of L. japonicus castor pollux double mutant by Lj-CASTOR^S266A, Lj-POLLUX^S329A, and DMI1^A294S.

(D) Lj-CASTOR^S266A rescues only Lj-castor.

(E) Lj-POLLUX^S329A gains function and rescues single as well as double mutant Lj-castor pollux.

(F) DMI1^A294S loses function and fails to rescue the Lj-castor pollux mutant.

In the figures, lowercase, italicized words correspond to the mutant line and uppercase, italicized words correspond to the respective gene or modified allele used for the rescue assay. Bars + 2 mm.

[See online article for color version of this figure.]