Table 2. Summary of Rescue Assay Performed with Different Constructs Expressing DMI1, Lj-CASTOR, Lj-POLLUX, Lj-CASTORS266A, Lj-POLLUXxS329A, and DMI11A294S in the dmi1-4 (GY15-3F-4) Mutant of M. truncatula.
| Construct | Mutant Line | Visible/Selection Marker | Nodulated Plants/Total Plants (Bump-Forming Plants/Total Plants) |
|---|---|---|---|
| 35Spro:DMI1 | dmi1-4a | GFP | 51/105 (15/105) |
| DMI1pro:DMI1 | dmi1-4 | DsRED1 | 45/89 (9/89) |
| 35Spro:DMI1A294S | dmi1-4 | GFP | 35/112 (12/112) |
| 35Spro:DMI1A294V | dmi1-4 | GFP | 0/93 |
| 35Spro:DMI1A294S +35Spro:Mt-CASTOR-RNAi | dmi1-4 | GFP +DsRED1 | 0/36 (21/36) |
| 35Spro:DMI1 + 35Spro:Mt-CASTOR-RNAi | dmi1-4 | GFP +DsRED1 | 20/20 |
| 35Spro:Lj-POLLUX | dmi1-4 | DsRED1 | 0/178 (1/178) |
| Lj-Ubq1pro:Lj-POLLUX | dmi1-4 | GFP | 0/81 (11/81) |
| Lj-POLLUXpro:Lj-POLLUX:RFPb | dmi1-4 | Kanamycin | 0/143 |
| DMI1pro:Lj-POLLUX | dmi1-4 | DsRED1 | 0/134 |
| 35Spro:Lj-POLLUXS329A | dmi1-4 | GFP | 76/171 (19/171) |
| 35Spro:Lj-CASTOR | dmi1-4 | GFP | 0/145 |
| Lj-Ubq1pro:Lj-CASTOR | dmi1-4 | GFP | 0/49 |
| Lj-CASTORpro:Lj-CASTOR:RFP | dmi1-4 | Kanamycin | 0/53 |
| DMI1pro:Lj-CASTOR | dmi1-4 | GFP | 0/81 |
| 35Spro:Lj-CASTORS266A | dmi1-4 | DsRED1 | 0/95 |
| 35Spro:Lj-CASTOR+35Spro:Lj-POLLUX | dmi1-4 | GFP +DsRED1 | 36/59 (13/59) |
| Lj-Ubq1pro:Lj-CASTOR + Lj-Ubq1pro:Lj-POLLUX | dmi1-4 | GFP | 55/64 (11/64) |
| 35Spro:Lj-CASTORS266A +35Spro:Lj-POLLUXS329A | dmi1-4 | GFP +DsRED1 | 16/28 (3/28) |
| 35Spro:Mt-CASTOR | dmi1-4 | GFP | 0/102 |
| DMI1pro:Mt-CASTOR | dmi1-4 | DsRED1 | 0/95 |
| 35Spro:At-POLLUX(ADSGSHA) | dmi1-4 | GFP | 0/40 |
| 35Spro:At-POLLUX(ADAGSHA) | dmi1-4 | GFP | 16/36 |
| 35Spro:At-POLLUX(ADAGNHA) | dmi1-4 | GFP | 14/36 |
a
dmi1-4 mutant line lacks full-length genomic region of DMI1.
b
RFP, red fluorescent protein.