Figure 4.

PP6-Dependent Dephosphorylation of PIN Proteins.

(A) In vitro kinase assay shows that the abundance of phosphorylated HIS-PIN2HL is higher when treated with plant extracts derived from PID-OE (POE), f1 f3, and F1DN seedlings compared with the treatments with plant extracts derived from Col and F1OE seedlings. WT, the wild type.

(B) In vitro kinase assay shows that the abundance of phosphorylated HIS-PIN2HL is higher when treated with plant extracts derived from F3Ri/f1 seedlings induced by ethanol than when treated with plant extracts derived from Col seedlings with or without ethanol induction and F3Ri/f1 seedlings without ethanol induction.

(C) Increased accumulation of higher molecular weight PIN2-GFP bands in f1 f3 roots compared with Col. These bands are sensitive to λ-phosphatase treatment but stable in the presence of phosphatase inhibitors. Mn²⁺ was added to each reaction to make the reaction buffer comparable. Ponceau S staining shows the loading control. PM, plasma membrane.

[See online article for color version of this figure.]