Figure 7.

Defective PIN1 and PIN2 Localization in the Roots of Ethanol-Induced ARS2 Transgenic Plants.

(A) to (C) After ethanol treatment, ARS2 transgenic plants ([B] and [C]) had shorter roots and agravitropic root growth as compared with Col (A).

(D) Dramatically reduced expression of SAL1, SAL3, and SAL4 mRNA in ARS2 lines after ethanol induction. Error bars represent se; n = 3.

(E) and (F) Immunolocalization of PIN1 in the 6-DAG roots of ARS2 lines after ethanol induction. PIN1 polar localization was disturbed, with visible apicalization in some stele cells of ARS2 roots compared with the basal localization of PIN1 in Col.

(G) and (H) Immunolocalization of PIN2 in 6-DAG roots of ARS2 lines after ethanol induction. In the Col background, PIN2 was localized to the upper side of the epidermal cells and the lower side of the cortical cells but shifted from basal to apical in the cortex of ARS2 roots. e, epidermal cells; c, cortical cells

Arrows indicate polarity of PIN localization. Bars = 2 cm in (A) to (C), 50 μm in (E) and (F), and 20 μm in (G) and (H).