Figure 1.

nas4x-2 Mutants Showed Multiple Morphological Phenotypes.

Growth phenotypes of wild-type and nas4x-2 plants during EVS, LVS, and RS growth stages. Plants were grown on soil under long-day conditions over a time course of up to 7 weeks (the wild type) and 9 weeks (nas4x-2). Note that nas4x-2 plants were growth retarded compared with the wild type.

(A) Photographs of morphological phenotypes. Bars = 1 cm.

(B) Schematic representation of the three growth stages analyzed. For molecular-physiological analysis, young expanding (y), middle-aged fully expanded (m), and aged, nearly senescent (a) leaves were harvested.

(C) Comparison of the leaf chlorosis phenotypes of nas4x-2 leaves at different growth stages, showing rescue of leaf chlorosis during maturation of the leaves. The different leaf chlorosis phenotypes are described, and the percentage of leaves manifesting these phenotypes is shown. Control, wild-type leaves of all stages were green (data not shown).

[See online article for color version of this figure.]