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. 2012 Jun 15;24(6):2380–2400. doi: 10.1105/tpc.112.099077

Figure 2.

Figure 2.

Young, Middle-Aged, and Aged Rosette Leaves of nas4x-2 Differed in Fe Content and in FER1 and YSL1 Expression.

Plants were grown on soil under long-day conditions up to EVS, LVS and RS; young (y), middle-aged (m), and aged (a) rosette leaves were harvested for analysis (see phenotypes in Figure 1).

(A) Fe contents of leaves, showing that Fe contents were reduced in young leaves of nas4x-2 plants, but increased in middle-aged and aged leaves of nas4x-2 in all analyzed stages (n = 4; *P < 0.05 for the comparison nas4x-2 versus the wild type). DW, dry weight.

(B) and (C) FER1 (B) and YSL1 (C) gene expression levels of rosette leaves, determined by quantitative RT--PCR, showing a reduction of gene expression in young leaves and an induction in aged leaves of nas4x-2 (error bars are sd; n = 3; *P < 0.05 for the comparison of nas4x-2 versus the wild type).