Figure 4.

The Double Loss of Function of FRD3 and NAS Epistatic and Synergistic Effects.

Growth phenotypes of wild-type, nas4x-2, frd3, and frd3 nas4x-2 plants grown on soil under long-day conditions up to EVS, LVS, and RS. Note that nas4x-2 and frd3 nas4x-2 plants were growth retarded.

(A) Photographs of morphological phenotypes showing that frd3 nas4x-2 quintuple mutants had a stronger leaf chlorosis than the other mutants. The images for the wild type and nas4x-2 are reproduced from Figure 1A. Bars = 1 cm.

(B) Comparison of the leaf chlorosis phenotypes of frd3 nas4x-2 leaves at different growth stages, showing the reduced rescue of leaf chlorosis during maturation of the leaves (cf. to nas4x-2 in Figure 1C). The different leaf chlorosis phenotypes are described, and the frequency of leaves manifesting these phenotypes is shown.

[See online article for color version of this figure.]