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. 2012 Jun 15;24(6):2380–2400. doi: 10.1105/tpc.112.099077

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© 2012 American Society of Plant Biologists. All rights reserved.

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Figure 5. — frd3 nas4x-2 Quintuple Mutants Had a Reduced Fe Content in Leaves and Reduced Concentrations of Citrate in the Xylem.

Plants were analyzed in the RS. Numbers 1 to 6 indicate P < 0.05 in the comparisons (1) nas4x-2 versus the wild type, (2) frd3 versus the wild type, (3) frd3 nas4x-2 versus the wild type, (4) frd3 versus nas4x-2, (5) frd3 nas4x-2 versus nas4x-2, and (6) frd3/nas4x-2 versus frd3.

(A) Fe contents of rosette leaves, showing that the elevated Fe content observed in the aged nas4x-2 leaves was alleviated in frd3 nas4x-2 (n = 4). DW, dry weight.

(B) Concentrations of citrate in the xylem sap, showing that nas4x-2 plants had an increased citrate concentration compared with the wild type, but not frd3 nas4x-2 plants (n = 5).