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. 2012 Jun 15;24(6):2380–2400. doi: 10.1105/tpc.112.099077

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© 2012 American Society of Plant Biologists. All rights reserved.

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Figure 6. — Fe Accumulated in the Vessels and in the Phloem in nas4x-2 Leaves.

Plants were analyzed in the RS.

(A) Perls Fe staining signals in leaves of the wild type and nas4x-2, showing Fe deposits in leaf veins (indicated by arrows) and hydathodes (marked by arrowheads) of nas4x-2 leaves; on the right side, unstained control. Further details are available in Supplemental Figure 2 online. Bar = 1 cm.

(B) Perls Fe staining signals in 7-µm sections of wild-type and nas4x-2 young and middle-aged leaves, showing Fe deposits in the phloem of the vessels in nas4x-2 (indicated by arrows); a magnification is shown in Supplemental Figure 5 online. Ph, phloem; Xy, xylem. Bars = 50 µm.