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. 2012 Jun 29;24(6):2352–2363. doi: 10.1105/tpc.112.100602

Table 3. Total Seed Number from Self-Pollination of Backcross 2 Plants Containing or Lacking the Designated Transgenic Event and Containing or Lacking the Endogenous Functional Sh2 Allele.

Event Parental Genotype First Planting Seed No. Ratio Second Planting
Seed No. Ratio Third Planting Seed No. Ratio Average Ratio
97 Addition 301* 1.23 302 1.08 407 1.71 337** 1.31
Substitution 338* 1.39 267 0.96 390 1.64 335** 1.31
Control 243 279 237 257
73 Addition 340** 1.61 303* 1.51 457 1.26 344* 1.26
Substitution 276 1.31 355 1.77 359 0.99 324 1.18
Control 211 200 362 274
133 Addition 375 0.93 503* 1.53 450 0.80 469 1.24
Substitution 360 0.89 521* 1.59 462 0.82 419 1.11
Control 408 329 562 377
183 Addition 414 1.14 450 1.20 414* 1.44 425** 1.25
Substitution 313 0.86 377 1.00 346 1.20 344 1.01
Control 364 376 287 340
114 Addition 314 0.98 416 0.97 411* 1.11 382 1.09
Substitution 327 1.02 373 0.87 352 0.95 354 1.01
Control 321 428 371 352
286 Addition 328 1.15 402* 1.35 466** 1.45 385** 1.30
Substitution 273 0.95 429** 1.43 397* 1.23 379** 1.28
Control 286 299 323 297
25 Addition 255 1.15 413** 1.48 389 1.37 360** 1.15
Substitution 290 1.31 381* 1.37 425 1.50 334** 1.31
Control 222 278 284 241
76 Addition 263 1.04 364 1.26 438* 1.32 351* 1.23
Substitution 259 1.02 341 1.18 383 1.15 338* 1.19
Control 252 290 332 284
Average Addition 330**c 1.19 380**b 1.24 426**a 1.31 378** 1.26
Substitution 304b 1.10 368**a 1.20 381**a 1.17 350** 1.17
Control 276b 307a,b 325a 299

The three genotypes are defined in Figure 1. Plants were grown in Citra, Florida, in the spring of 2005. Plantings were done weekly over 3 weeks and are labeled first, second, and third planting. Total seed numbers marked with asterisks designate significant differences at the 5% (*) or 1% (**) level from the cognate nontransgene control of the same planting date. The ratio is the seed number of each of the two transgene-containing genotypes divided by the seed number of the nontransgenic control that contains one copy of the functional Sh2 allele. Letters refer to significant (5%) differences distinguishing the three planting dates. Averages and ratios of all events were calculated from all individual data. The total population was 498 ears and 170,448 kernels.