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. 2012 Jun 22;24(6):2546–2561. doi: 10.1105/tpc.112.100107

Figure 3.

Figure 3.

Genetic Analysis of ghr1 with ost1 Suggests That GHR1 Is in Parallel with OST1 and/or Acts Downstream of OST1.

(A) Drought phenotypes of wild-type (WT), ost1, ghr1, and ghr1 ost1 seedlings in soil after water was withheld for 15 d (left) or 20 d (right).

(B) Water loss of wild-type, ghr1, ost1, and ghr1 ost1 seedlings growing in soil as determined by the weighing of pots over a 24-h period during the drought treatment in (A). The values in the top panel are means of three replicates in one experiment; each replicate was represented by one pot, and each pot was weighed every 5 min. The values in the bottom panel are means of three replicate pots that were weighed each hour (by reducing the data, the bottom panel more clearly reveals the trends). At all time points, water loss was significantly greater (P < 0.01) for ghr1 ost1 than for the wild-type, ghr1, and ost1 seedlings. At all time points of night (without light), water loss was significantly greater (P < 0.05) for ghr1 than for the wild type or ost1, while water loss did not differ between the wild type and ost1. In the period simulating day (with light), water loss was significantly greater (P < 0.05) for ghr1 than for the wild type.

(C) Sizes of stomatal apertures in the dark. Epidermal peels with preopened stomata were kept in the dark for different times before the apertures were measured. Values are means ± se of three replicates (30 stomata from one seedling per replicate) for one experiment (**P < 0.01, *P < 0.05).

(D) Water loss of detached leaves of the wild type, ghr1, ost1, and ghr1 ost1. For the quantification of water loss from detached leaves, three independent experiments were done with similar results. Values are means ± se of three replicates (40 leaves from one pot were measured per replicate) for one experiment. Water loss was significantly greater for ghr1 ost1 than for ost1 or ghr1 from 2 to 6 h (**P < 0.01, *P < 0.05).

(E) ABA-induced stomatal closure in the wild type, ghr1, ost1, and ghr1 ost1. Values are means ± se of three replicates (30 stomata from one seedling per replicate) from one experiment; three independent experiments were performed with similar results. Stomatal apertures were significantly greater (**P < 0.01) for ghr1 ost1 than for the wild type, ghr1, or ost1.