Figure 4.

Genetic Analysis of ghr1 with abi1-11; abi2-1 Dominant Negative Mutants; abi1, abi2, and hab1 Null Mutants; and the slac1 Mutant.

(A) Water loss of detached leaves of the wild type (WT), ghr1, abi2-1 (a dominant negative mutation; Landsberg erecta), and ghr1 abi2-1.

(B) Water loss of detached leaves of the wild type, ghr1, abi1-11 (a dominant negative mutation; Col-0), and ghr1 abi1-11.

(C) Water loss of detached leaves of the wild type, ghr1, abi1 abi2 double mutant, ghr1 abi1 abi2 triple mutant, abi1 abi2 hab1 triple mutant, and ghr1 abi1 abi2 hab1 quadruple mutant. abi1 (Salk_072009), abi2 (Salk_015166), and hab1 (Salk_002104) are loss-of-function mutants with a T-DNA insertion in each gene.

(D) Water loss of detached leaves of the wild type, ghr1, slac1-4, and ghr1 slac1-4.

(E) Water loss of detached leaves of the wild type, ghr1, ost1, slac1-4, and ghr1 ost1.

For the quantification of water loss from detached leaves, three independent experiments were done with similar results. Values are means ± se of three replicates (40 leaves from one pot were measured per replicate). Water loss was not significantly different between abi1-11 ghr1 and abi1-11 (A), between abi2-1 ghr1 and abi2-1 (B), between ghr1 and ghr1 abi1 abi2 hab1 or ghr1 abi1 abi2 (C), or between slac1-4 and ghr1 slac1-4 (D). In (E), water loss was significantly greater for ghr1 ost1 than for slac1-4 from 1.5 to 4 h (*P < 0.05, **P < 0.01). Error bars are smaller than symbols when not visible.