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. 2012 Jun 19;24(6):2427–2442. doi: 10.1105/tpc.112.098723

Figure 7.

Figure 7.

Functional Relevance of CCA1β in Circadian Rhythms.

(A) Circadian rhythms of GI and LHY gene expression in CCA1pro:CCA1α cca1-2 plants that lack functional CCA1β. In the CCA1pro:CCA1α cca1-2 plants, CCA1α expression was driven by the endogenous CCA1 promoter in the cca1-2 mutant background. Plants grown on MS-agar plates under neutral day cycles for 10 d were transferred to continuous light conditions. Whole plants were harvested at ZT points up to 48 h, and gene transcript levels were determined by qRT-PCR. Biological triplicates were averaged. Bars indicate the se.

(B) Quantification of CCA1α and CCA1β transcripts. Two-week-old Col-0 plants grown on MS-agar plates were harvested at ZT4 and used for extraction of total RNA. A series of 10-fold dilutions of the CCA1 plasmid DNA was used to draw the absolute standard curve. The regression line from the dilution curve was used to determine the concentration of CCA1α and CCA1β. Red squares represent the absolute amount of CCA1α and CCA1β transcripts. Ct, threshold cycle.