Figure 11.
Increased SOD Activity in Fe-Starved Cells.
Strain CC425 was grown in TAP medium with 18 μM Fe, washed, inoculated into TAP medium with 0 μM added Fe, and allowed to grow for 5 d. Data from three individual experiments are shown, represented in (A) to (C) by circles, triangles, and squares.
(A) Total soluble SOD activity per cell, normalized to day 0. SOD activities at day 0 were 1.95, 1.59, and 1.46 units per 106 cells for the three replicates.
(B) SOD specific activities in soluble protein extracts, normalized to day 0. SOD specific activities at day 0 were 39.2, 31.6, and 28.5 units per mg protein for the three replicates.
(C) Protein concentration in soluble extracts, normalized to day 0.
(D) Abundance of FeSOD. Soluble proteins (1 μg) were separated by denaturing gel electrophoresis and transferred to nitrocellulose for immunoblot analysis.