Figure 3.

A Novel SOD Induced under Fe Limitation Is Expressed Coordinately with the MSD3 Gene.

C. reinhardtii strain CC125 was grown in TAP medium containing the indicated Fe supplement.

(A) Soluble proteins (60 μg) were separated by nondenaturing gel electrophoresis and SODs visualized by activity staining (top panel). Total proteins (20 μg) were separated by denaturing gel electrophoresis and transferred to PVDF or nitrocellulose for immunoblot analysis (bottom three panels).

(B) RNA was analyzed by quantitative real-time PCR for the abundance of transcripts encoding MSD1, MSD2, and MSD3. Primers used are listed in Supplemental Table 5 online. The fold difference in abundance of each mRNA is shown after normalization to CBLP. Error bars represent the variation (sd) between experimental triplicates.