Figure 6.

MSD3 Is Induced by Fe Limitation, Mn Deficiency, or H₂O₂ Stress.

RNA from Fe-deficient (closed circles) and Mn-deficient (open circles) cells and from cells treated with 1 mM H₂O₂ (triangles) was analyzed by quantitative real-time PCR for the abundance of transcripts encoding FSD1 and MSD1 through MSD5. Primers used are listed in Supplemental Table 5 online. C. reinhardtii strains 2137, CC125, and CC425 were grown in TAP medium with either 0.5 or 18 μM added Fe and either 0 or 25 μM added Mn to ∼3 × 10⁶ cells/mL. For H₂O₂ treatment, cells were grown to ∼2 × 10⁶ cells/mL, H₂O₂ added to 1 mM final concentration, and cells harvested 2 h later. This concentration of H₂O₂ was chosen based on previous studies (Leisinger et al., 2001).The fold difference in abundance of each mRNA is shown after normalization to CBLP. Each data point is the average of technical triplicates and represents an individual experiment.