Figure 8.

Identification of MnSOD3 as the MSD3 Gene Product.

Soluble proteins (60 and 20 μg) isolated from cells grown under conditions of Fe repletion (18 μM Fe) or starvation (0 μM added Fe, 5 d) were separated by nondenaturing gel electrophoresis and the gel divided in three. For one part of the gel (60 μg protein), SODs were visualized by activity staining; for the other parts (20 μg protein), proteins were transferred to nitrocellulose for immunoblotting with anti-MnSOD3 (1:1000) or anti-FeSOD (1:2000).