Figure 5.

EIN3 Negatively Regulates the Expression of Cold-Regulated Genes in the CBF Pathway.

(A) Expression of CBFs and their regulons in wild-type and ein3 eil1 seedlings at 22 and 4°C. Two-week-old seedlings grown at 22°C were treated at 4°C for the indicated periods of time. The data are the mean values of three replicates ± sd.

(B) Expression of CBF genes in the iE/qm transgenic plants with or without 5 μM β-estradiol treatment for 4 h, followed by cold treatment at 4°C for the indicated period of time. The data represent the mean values of three replicates ± sd.