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. 2012 Jun 22;24(6):2515–2527. doi: 10.1105/tpc.112.099119

Figure 3.

Figure 3.

GH3.3, GH3.5, and GH3.6 Regulate Adventitious Root Initiation by Modulating JA Homeostasis.

(A) to (C) Endogenous free IAA (A), free SA (B), and free JA (C) contents were measured in the hypocotyl of wild type ecotype Columbia (Col-0) and the gh3.3-1gh3.5-2gh3.6-1 triple mutant. Seedlings were first etiolated in the dark, until their hypocotyls were 6 mm long (T0), and then transferred to the light for 72 h (T72L). Error bars indicate sd of eight biological replicates. A one-way analysis of variance combined with the Bonferroni posttest indicated that the values indicated by asterisks are significantly different from wild-type Col-0 values and the values indicated by hash signs are significantly different from values observed at T0 (P < 0.05; n = 8). FW, fresh weight.

(D) and (E) Endogenous free JA (D) and JA-Ile (E) were measured in the hypocotyl of wild type Col-0 and the gh3.3-1gh3.5-2gh3.6-1 triple mutant, etiolated as in (A) (T0) or transferred to the light for 3 h (T3L) or 9 h (T9L). Error bars indicate sd of nine biological replicates. A one-way analysis of variance combined with the Bonferroni posttest indicated that the values indicated by asterisks are significantly different from wild-type Col-0 values (P < 0.05; n = 9).