Figure 4.

GH3.3, GH3.5, and GH3.6 Can Conjugate JA to Amino Acids in Vitro.

Thin layer chromatography analysis of JA-amino acid conjugates synthesized by recombinant GST:GH3.3, GST:GH3.5, and GST:GH3.6 proteins. Substrates added to each enzyme reaction mixture are indicated below the image. Concentrations for JA and IAA were 10 and 1 mM, respectively. JA conjugate standards, shown to the right, migrated with somewhat greater mobility because they were loaded on the plate in organic solvent rather than the enzyme reaction mixture. Free IAA and JA migrated to near the top of the plate.