Figure 2.
Histochemical Analysis of PME35 Expression and Lignin Deposition Pattern.
(A) and (B) Cross sections derived from the upper part of the inflorescence stem of a ProPME35:GUS plant.
(C) A GUS-stained section of the upper part of the inflorescence stem of a ProPME35:GUS plant was further stained with phloroglucinol-HCl to disclose lignin deposition in red.
(D) and (E) Cross sections derived from the middle part of the inflorescence stem of a ProPME35:GUS plant.
(F) A GUS-stained section of the middle part of the inflorescence stem of a ProPME35:GUS plant was further stained with phloroglucinol-HCl.
(G) and (H) Cross sections derived from the basal part of the inflorescence stem of a ProPME35:GUS plant.
(I) A GUS-stained section of the basal part of the inflorescence stem of a ProPME35:GUS plant was further stained with phloroglucinol-HCl.
co, cortex; if, interfascicular fiber; xy, xylem. Bars = 100 µm in (A), (D), and (G) and 20 µm in (B), (C), (E), (F), (H), and (I).
