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. 2012 Jun 26;24(6):2696–2706. doi: 10.1105/tpc.112.098954

Table 1. Kinetic Comparison of Gm-CAS and Gm-OASS Substrate Specificities.

Enzyme	CAS Reaction Substrates			OASS Reaction Substrates
	Cys			O-acetylserine
	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (m⁻¹ s⁻¹)	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (m⁻¹ s⁻¹)
Gm-CAS	38.9 ± 4.5	0.81 ± 0.25	48,024	1.82 ± 0.09	8.87 ± 1.31	205
Gm-OASS	0.21 ± 0.09	0.30 ± 0.01	700	57.5 ± 11.8	3.60 ± 0.40	15,972
	KCN			Na₂S
	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (m⁻¹ s⁻¹)	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (m⁻¹ s⁻¹)
Gm-CAS	39.2 ± 0.03	0.26 ± 0.03	150,769	1.78 ± 0.13	0.11 ± 0.06	16,182
Gm-OASS	0.23 ± 0.03	0.08 ± 0.02	2,875	39.2 ± 5.7	0.05 ± 0.02	784,000

All values are expressed as a mean ± se (n = 3).