. 2012 Jun 27;12:33. doi: 10.1186/1472-6750-12-33

Table 2.

Sample preparation procedure and 454 sequencing results

Sample ID	Preparation steps	# raw reads	# reads in contigs	# contigs	# singletons	% globin reads
A1	ribo-minus > 1^st & 2^nd strand cDNA synthesis > NORM > 2^nd amp	30,145	9,138	1,066	19,205	5.46
A2	wholeRNA > 1^st&2^nd strand cDNA synthesis > 2^nd amp > NORM > 3^rd amp	38,601	19,318	2,286	14,079	1.95
B1	globin-minus > ribo-minus > 1^st&2^nd strand cDNA synthesis	21,219	13,075	275	5,987	24.10
B2	globin-minus > ribo-minus > 1^st&2^nd strand cDNAsynthesis > NORM > 2^nd amp	17,047	4,391	309	11,168	0.35

# counted.

Abbreviations used for preparation steps are explained in the Supplementary Information Additional files.