Figure 4.

Proliferation of CD8 + IFNγ + cells from LSIL patients (LSIL), and normal donors (Normal) after stimulation with phytohemagglutinin (PHA). PBL from LSIL patients were cultured for 3 days in the presence of PHA, and then analysed for surface CD8 and intracellular IFNγ expression. The proportion of CD8 + IFNγ + stimulated by PHA were evaluated by flow cytometry. A) shows a representative plot obtained from PBL of a LSIL patient incubated with phytohemagglutinin (PHA) and in absence of PHA, as a control (−). B) shows a representative plot obtained from PBL of a normal donor incubated with phytohemagglutinin (PHA) and in absence of PHA (−). Bars in C) depict the mean of CD8 + IFNγ + percentages for each set of samples. Error bars indicate the standard error of the mean. A statistically significative difference was found between the groups with a p < 0.05*.