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. 2012 May 29;7:12. doi: 10.1186/1750-9378-7-12

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Copyright ©2012 Hernandez-Montes et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License(http://creativecommons.org/licenses/by/2.0),which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Proliferation of PBL from LSIL patients in response to HPV antigens presented by monocytic (a,b) and by dendritic cells (c,d). PBL from two LSIL patients, infected by HPV-16 (P1), and HPV-18 (P2) were incubated with Cervarix®, that contains capsid antigens of HPV-16, and HPV-18, to evaluate the induced proliferation. Carboxifluorescein (CFSE) labeled PBL from patients P1 and P2 were incubated in the presence of soluble Cervarix® (PBL + Cervarix) (b), and PBL alone were used as a control (PBL) (a). CFSE labeled PBL from patients P1 and P2 were cultured with autologous dendritic cells (PBL + DCs), previously “loaded” with Cervarix® (d), or with autologous unloaded dendritic cells as a control (PBL + DCs/Cvx) (c). The percentage of decrease in carboxiflourescein label, as an indicator of cell proliferation is shown under the bar (M1) of each histogram.