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. 2012 Jul 3;9:23. doi: 10.1186/1743-8977-9-23

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Copyright ©2012 Freese et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Internalization of gold nanoparticles in HDMEC and hCMEC/D3 analyzed by transmission electron microscopy. HDMEC (A-C) and hCMEC/D3 (D-F) were incubated with 300 μM gold nanoparticles for 24 hours. After exposure, cells were extensively washed, fixed with paraformaldehyde and examined by transmission electron microscopy (TEM). AuS0302-RIT, AuS0302-RIS02 and AuS0302-RIS04 were found in intracellular vesicles which were mostly located in the perinuclear region. The arrow heads indicate the gold nanoparticles within the vesicles. Scale bar: 1 μm.