Table 1.
Solvent systems for TLC analysis of mycobacterial lipids (adapted from[22]).
| Solvent System | Run Direction | Components | Runs | Lipids Resolved |
|---|---|---|---|---|
| A |
1 |
petroleum ether : ethyl acetate (98 : 2) |
3 |
PDIM, TAG, MQ |
| |
2 |
petroleum ether : acetone (98 : 2) |
1 |
|
| B |
1 |
petroleum ether : acetone (92 : 8) |
3 |
AT, FA |
| |
2 |
toluene : acetone (95 : 5) |
1 |
|
| C |
1 |
chloroform : methanol (96 : 4) |
1 |
FA, GLY |
| |
2 |
toluene : acetone (80 : 20) |
1 |
|
| D |
1 |
chloroform : methanol : water (100 : 14 : 0.8) |
1 |
CF, SL, DAT |
| |
2 |
chloroform : acetone : methanol : water (50 : 60: 2.5 : 3) |
1 |
|
| E |
1 |
chloroform : methanol : water (60 : 30 : 6) |
1 |
DPG, PE PI, PIM |
| 2 | chloroform : acetic acid : methanol : water (40 : 25 : 3 : 6) | 1 |