Table 1.

Primer sequences used in this study

Real-time PCR analysis
Target gene	Forward (5′ → 3′)	Reverse (5′ → 3′)	Probe (5′ → 3′)
MCPyV ST	GCAAAAAAACTGTCTGACGTGG	CCACCAGTCAAAACTTTCCCA	FAM-TATCAGTGCTTTATTCTTTGGTTTGGATTTCCTCCT- TAMRA
HPyV9 VP1	TGCTGTTGATATTGTTGGAATTCA	AACAACCCGTTTCCTTAGAGTTACA	FAM-CTGGAGAGGCCTACCT-NFQ-MGB
Human RNase P	AGATTTGGACCTGCGAGCG	GAGCGGCTGTCTCCACAAGT	FAM-TTCTGACCTGAAGGCTCTGCGCG-TAMRA
PCR analysis
Target gene (positions)	Forward (5′ → 3′)	Reverse (5′ → 3′)
MCPyV LT (1867–2221)	AGCCCCCTTACAAATTACTGCAAG*	AGCATTTCTGTCCTGGTCATTTC*
MCPyV ST-LT (183–828)	GCATATAGACAAGATGGATTTA†	ATAACCTTTCTTTGATATTTTGC†
MCPyV ST-LT (571–1157)	TTGTCTCGCCAGCATTGTAG†	GGATCCAGAGGATGAGGTGGGTTC†

^*Primers for mutational analysis.

^†Primers for sequence analysis of the ST-LT genes.