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Kinetic Parameters for Reaction With D- and L-GpNA.
| Kinetic parameter | D-GpNA Minus acceptor | D-GpNA Plus acceptor | L-GpNA Minus acceptor | L-GpNA Plus acceptor |
|---|---|---|---|---|
| V (mM/min/nM) | 2.1 ± 0.2 | 53 ± 11 | 6.5 ± 0.2 | 141 ± 11 |
| V/Kglygly (min-1nM-1) | 7.6 ± 1.5 | 14 ± 1 | ||
| V/KGpNA (min-1nM-1) | 13.2 ± 0.1 | 12.7 ± 2.5 | 7.8 ± 0.2 | 117 ± 9 |
| Kglygly (mM) | 7.0 ± 0.3 | 9.9 ± 0.9 | ||
| KGpNA (mM) | 0.16 ± 0.02 | 4.2 ± 0.1 | 0.83 ± 0.06 | 1.2 ± 0.1 |
aThe acceptor is glygly.
