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. 2012 Jul 27;7(7):e42050. doi: 10.1371/journal.pone.0042050

Figure 2. Expression and purification of SjGALE in E. coli.

Figure 2

Cell extracts and fractions from E. coli BL21 (DE3) transformed with the pET28-SjGALE were separated by 12% SDS-PAGE. Lanes 1 and 2: total extract of a clone before and after induction with 1 mM of IPTG. Lanes 3 and 4: supernatant and inclusion bodies after lysis, respectively. Lane 5: the protein in supernatant purified through Ni-NTA His-Bind resin. Lane 6: western blot profile (antigen: native protein of S. japonicum and primary antibody: polyclonal anti-rSjGALE antibody).