Figure 4. Western blot analysis of the SjGALE protein level at different stages of the S. japonicum life cycle.

Total protein lysates from different stages of S. japonicum as indicated were separated on 12% polyacrylamide gels and electrophoretically transferred to nitrocellulose filters. Blots were probed with mouse anti-SjGALE and anti-α-tubulin as a loading control. (A) Tubulin was used as a control to determine the sample preparation. (B) Extracts were prepared from various stages of the S. japonicum life cycle and subjected to immunoblotting with anti-rSjGALE serum. (C) The western blot bands were converted into a histogram by measuring the optic density of the autoradiogram band.