Figure 5. Immunolocalization of SjGALE from adult male and female S. japonicum.

Polyclonal anti-rSjGALE antibody was used as primary antibody and serum from naive mice was used as a negative control. Alexa Flour 488-conjugated (green) anti-mouse IgG was used as a second antibody. DAPI was used to stain nuclei (blue). (A): male adult worm. (C): male and female paring worms. (E): negative control. (B, D and F): DIC images of A, C, E, separately. (G): Detail of the image from panel (C) for detection of SjGALE (60× magnification).