Table 1. Oligonucleotides used in EMSA.

Oligo	Sequence	Position relative to TSS
LKB1 FOXO Wt	5′-GGGGAGGGAGGTAAACAAGATGGCGGC-3′	−27 to −1
LKB1 FOXO Mut	5′-GGGGAGGGAGGTAGCCAAGATGGCGGC-3′	−27 to −1
FOXO3 FOXO A	5′-AGCCACCGCAGTAAGCAGTCAGAGCCC-3′	−2439 to −2413
FOXO3 FOXO B	5′-TGGGCGAAGCACAAACAGGAGGCTTTG-3′	−2168 to −2142
FOXO3 FOXO C	5′-CATTCCATTTAAAAATAACACCCACGT-3′	−1865 to −1839
FOXO3 FOXO D	5′-ACGTGCGCTGGTAAACAAGCGCGCGCG-3′	−1842 to −1816
FOXO E1	5′-TTTACTCGATTGTTTATTATTTGCTGG-3′	−1446 to −1420
FOXO E2	5′-GATTGTTTATTATTTGCTGGGGGGCGG-3′	−1439 to −1413
FOXO F	5′-TGACATCATCTGTTTGTACGCGTCGAA-3′	−1270 to −1244
FOXO G	5′-TTAAAAAATATATTTATGAACTAGTGT-3′	−1059 to −1033
FOXO H	5′-CTTGTGCAGATGTTTTTCGTTTAACCT-3′	−949 to −923
FOXO3 FOXO E2 WT	5′-TAGAATTTTACTCGATTGTTTATTATTTGCTGGGGGGCGG-3′	−1452 to −1413
FOXO3 E2 Linker	5′-GATTGTTTAT TAGCGCGGCGCGTTATTTGCTGGGGGGCGG-3′	−1452 to −1413
FOXO3 E2 MUT	5′-TAGAATTTTACTCGATTGCGTATTACGTGCTGGGGGGCGG-3′	−1452 to −1413

FOXO3 core binding motive is indicated in bold. Parts of adjacent FOXO3 binding sites within the same oligonucleotide are underlined.