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The sperm lysed with trypsin were treated with A: 0.05 or B: 0.1 mol/L NaOH prior to SCPFGE, C: treated with 0.1 mol/L NaOH after electrophoresis, and D: the sperm lysed without trypsin were treated with 0.1 mol/L NaOH prior to SCPFGE.
