Table 1. Oligonucleotides used for PCR and cloning.
Gene | Acc.No | Comment | Forward (3′–5′) | Reverse (3′–5′) |
LYL1 | NM_005583 | ChIP | GAGGCCCGCGCTGCTAGGTC | CCCGGTTTCCTCCCTCTCAC |
MSX2 | XM_037646 | RQ-PCR | AGATGGAGCGGCGTGGATGC | CTCTGCACGCTCTGCAATGG |
NKX3-1 | AF247704 | ChIP | GGCATTTCTAAACATGTCCAGCTGC | CTACCTCTCCCAGGACTGGG |
reporter | CTAAGCTTGTCCTCTGAGTTGCCCAGTC | TTGGATCCGGGAGGATATTAATGTTCTGTTTC | ||
SIX6 | NM_007374 | reporter | CAAAGCTTACAAATACAGAGGGCATTTGG | ATGGATCCCTGTTCCTTGCACAGTGCCTG |
TBP | NM_001172085 | RQ-PCR | CGGAGAGTTCTGGGATTGT | CACGAAGTGCAATGGTCTTT |
Restriction sites for HindIII and BamHI are underlined.