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. 2012 Jul 27;7(7):e40747. doi: 10.1371/journal.pone.0040747

Table 1. Oligonucleotides used for PCR and cloning.

Gene Acc.No Comment Forward (3′–5′) Reverse (3′–5′)
LYL1 NM_005583 ChIP GAGGCCCGCGCTGCTAGGTC CCCGGTTTCCTCCCTCTCAC
MSX2 XM_037646 RQ-PCR AGATGGAGCGGCGTGGATGC CTCTGCACGCTCTGCAATGG
NKX3-1 AF247704 ChIP GGCATTTCTAAACATGTCCAGCTGC CTACCTCTCCCAGGACTGGG
reporter CTAAGCTTGTCCTCTGAGTTGCCCAGTC TTGGATCCGGGAGGATATTAATGTTCTGTTTC
SIX6 NM_007374 reporter CAAAGCTTACAAATACAGAGGGCATTTGG ATGGATCCCTGTTCCTTGCACAGTGCCTG
TBP NM_001172085 RQ-PCR CGGAGAGTTCTGGGATTGT CACGAAGTGCAATGGTCTTT

Restriction sites for HindIII and BamHI are underlined.