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. 2012 Jul 27;7(7):e41033. doi: 10.1371/journal.pone.0041033

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Forgione, Tropepe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Iba1 positive microglial cell displaying a ramified morphology. (B) Iba1 positive amoeboid microglia. (C) Iba1 positive cells in an untreated, control midbrain culture. (D) Treatment with 50 mM SB for 48 hours significantly reduces the number of Iba1 positive cells in midbrain cultures. (E) Quantification of the decrease in Iba1 positive cells as a result of HDACi treatment. Bars represent the mean + s.d. (n = 4). Asterisk (*) indicates that SB only treatment group is significantly different (p<0.05) from the control treated group as determined by an unpaired Student’s t-test. Scale bars = 50 µM. Blue = DAPI positive nuclei, green = Iba1.