Table 1. qPCR primer sequences and expected amplicon size.
Gene Name | Forward Primer | Reverse Primer | Amplicon (bp) |
GAPDH | GGTGCTGAGTATGTCGTGGA | CCTTCCACAATGCCAAAGTT | 284 |
GFAP | ACAAATCACTTCCTTCATCC | TAGAGAGACTTTGCCTCAGG | 125 |
Nefm | GTGAAGTCTCCTGAGGCTAA | TCTGCCTGGTCTGACTTGAC | 113 |
Hoxa10 | CCAGCCCTGGGTAAACTTAGC | CGGCTCCTTGCACCATTG | 87 |
TLR4 | AATACAAGCCATGTCATGTT | GGAATGAAGACCTCTCAAAA | 140 |
Robo3 | GAATCGCCGAGAGGAACCAA | ACATCGGTTGACCAGGGAAG | 100 |
Nurr1 | CCTGTCAGCACTACGGTGTT | CTTGTCCACTGGGCAGTTTT | 116 |