Table 1. Sequences of oligonucleotide primers used for real-time PCR, length of the PCR products and the accession numbers.

Gene	Primer sequences (5′to3′)		Product size (bp)	Accession number	Ref.
HRRT	F	GTGATAGATCCATTCCTATGACTGTAGA	104	U69731	[60]
	R	TGAGAGATCATCTCCACCAATTACTT
TLR2	F	ACATGAAGATGATGTGGGCC	109	AB072190	[61]
	R	TAGGAGTCCTGCTCACTGTA
TLR4	F	GCCATCGCTGCTAACATCATC	108	NM_001113039	[61]
	R	CTCATACTCAAAGATACACCATCGG
TLR5	F	CAGCGACCAAAACAGATTGA	122	NM_001123202	[52]
	R	TGCTCACCAGACAGACAACC
TLR9	F	GTGGAACTGTTTTGGCATC	199	NM_213958.1	[62]
	R	CACAGCACTCTGAGCTTTGT
NOD1	F	ACCGATCCAGTGAGCAGATA	140	NM_001114277	[52]
	R	AAGTCCACCAGCTCCATGAT
NOD2	F	GAGCGCATCCTCTTAACTTTCG	66	NM_001105295	[20]
	R	ACGCTCGTGATCCGTGAAC
IL-8	F	TCCTGCTTTCTGCAGCTCTC	176	NM_213867	[63]
	R	GGGTGGAAAGGTGTGGAATG
TNF-α	F	GCCCACGTTGTAGCCAATGTCAAA	99	NM_214022	[64]
	R	GTTGTCTTTCAGCTTCACGCCGTT
pBD-2	F	CTGTCTGCCTCCTCTCTTCC	148	NM_214022	[52]
	R	CAGGTCCCTTCAATCCTGTT

HPRT =  hypoxanthine phosphoribosyl-transferase; TLR  =  Toll-like receptor; NOD  =  nucleotide binding oligomerization domain; IL  =  interleukin; TNF  =  tumor necrosis factor; pBD-2 =  porcine β-defensin-2; F  =  forward primer; R  =  reverse primer; Ref.  =  reference.