Figure 1. qRT-PCR validation of selected differentially expressed genes identified by microarray.

Quantitative PCR on a panel of 7 genes was used to verify the transcriptional changes observed by microarray analysis. Consistent with the microarray results, expression of PPARA, RHOD, ABCG8, PPARG and ADA was upregulated when comparing infected animals to controls, while a downregulation could be seen for RASGRP2 (although not significant) and FASN. (* P<0.05).