Figure 1. Inducible targeting of the Fbxw7 gene in retinal ECs.
Confocal images showing the organization of the Fbxw7 iECKO mutant and littermate control retinal vasculature at postnatal day 6 (P6) after whole-mount Isolectin B4 staining (A and C). Postnatal, EC-specific loss-of-function mutants were either generated with the Cdh5(PAC)-CreERT2 (A, B) or Pdgfb-iCre (C, D) transgenics. Radial extension, branchpoint number, sprouts, and vascular area (B and D) were quantitated (control, n = 7; Fbxw7 iECKO n = 6). Error bars indicate SEM. P value (***) <0.00001. Scale bar is 200 µm for all microscopic images.
