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CK+: pBI 121-OsAPx2 plasmid, CK-: untransformed alfalfa, 1–7: transgenic alfalfa with rice OsAPx2. The volume was diluted up to100 then measured in a pipette with 1 µL for the PCR template. The result shows that 48 amplification products by PCR are identified with pBI121-OsAPx2’s (750 bp), and none of the products was identified with negative blank (ck-).
