Table 2. HCV NS3-specific UDPS-primers.
Genomic region and primer | Sequence | Position* | Amplicon coverage (bp) |
Amplicon NS3-I: V36/T54 | |||
VX1-Sense | 5′- 454A-MID- AGCYTIACYGGCCGRGA - 3′ | 3477–3493 | 181 |
VX1-Antisense | 5′- 454B-MID- TGGTCYACATTGGTRTACATYTG - 3′ | 3636–3658 | |
Amplicon NS3-II: R155/A156 | |||
VX2-Sense | 5′- 454A-MID- CAYGCYGATGTCATYCC -3′ | 3747–3763 | 298 |
VX2-Antisense | 5′- 454B-MID- CCRCTICCIGTRGGRGC - 3′ | 4029–4045 |
Relative to H77 (GenBank accession number AF00960).