Table 4. HCV-1b NS3 UDPS mutation analysis.

	HCV-1b Amplicon NS3-I								HCV-1b Amplicon NS3-II
	V36				T54				R155				A156
ID	Baseline		Follow-up		Baseline		Follow-up		Baseline		Follow-up		Baseline		Follow-up
4	0.02 A	(16899)	0	(7312)	0	(16564)	0	(7224)	0.02 S	(4500)	0	(2459)	0.02 G	(4523)	0.04 D; 0.04 V	(2459)
5	0.39 I	(15804)	0	(13911)	0.02 I	(15191)	0	(13333)	0	(7363)	0	(5005)	0.04 T	(7361)	0	(5004)
7	0	(10184)	0	(88567)	0	(10068)	A 0.02	(86402)	0	(3030)	0.03 W	(7924)	0	(3029)	0.05 D	(7916)
9		NA		NA		NA		NA	0	(988)	0	(3046)	0	(988)	0	(3045)
12	0	(10433)	0.03 G	(3927)	0.54 A	(10257)	0	(3869)	0.72 P	(2340)	0	(1253)	0.68 G	(2340)	0	(1253)
14	0	(13875)	0	(8714)	0	(13218)	0	(8129)	0.03 W	(7180)	0	(20478)	0	(7180)	0	(20466)

Percentage of UDPS reads with mutations. The parenthetical values indicate the number of reads analyzed. The bold numbers indicate variation present at a frequency above 0.1%. Underlined amino acids indicate resistant variants that were also observed by clonal sequencing at EOT. UDPS of both fragments (NS3-I, NS3-II) failed for patient 6 at both baseline and follow-up.