Table 2. Gradient elution program for HPLC-ELSD separation.
Time (min) | solvents | Flow rate (ml/min) | ||
A(%) | B(%) | C(%) | ||
0 | 100 | 0 | 0 | 1.0 |
4 | 100 | 0 | 0 | 1.0 |
5 | 85 | 15 | 0 | 1.0 |
10 | 80 | 20 | 0 | 1.0 |
12 | 75 | 25 | 0 | 1.0 |
15 | 50 | 50 | 0 | 1.0 |
18 | 30 | 50 | 20 | 1.0 |
20 | 30 | 40 | 30 | 1.0 |
25 | 25 | 30 | 45 | 1.0 |
30 | 30 | 70 | 0 | 1.0 |
40 | 100 | 0 | 0 | 1.0 |