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. 2012 Jul 27;7(7):e38689. doi: 10.1371/journal.pone.0038689

Table 2. Gradient elution program for HPLC-ELSD separation.

Time (min) solvents Flow rate (ml/min)
A(%) B(%) C(%)
0 100 0 0 1.0
4 100 0 0 1.0
5 85 15 0 1.0
10 80 20 0 1.0
12 75 25 0 1.0
15 50 50 0 1.0
18 30 50 20 1.0
20 30 40 30 1.0
25 25 30 45 1.0
30 30 70 0 1.0
40 100 0 0 1.0