Figure 1. Designed and SELEX selected duplexes tested for binding to RTs.
Shown is the sequence of the duplex constructs tested for binding to the various RTs (see Table 1). Each template strand was 45 nts in length while primer strands were 41 nts. All constructs had a four nt 5′ overhang. Capital letters are sequences that were derived from the PPT containing region of the genome for the particular designed duplex (other than the Random duplex) or those that were selected from the randomized region for the SELEX-derived duplexes. The sequences with small lettering are identical in all the constructs and were derived from the fixed primer region in the primer-template SELEX protocol.