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. 2012 Jul 27;7(7):e38375. doi: 10.1371/journal.pone.0038375

Figure 1. CsA promotes CXCL12 secretion in primary human trophoblast cells and JEG-3 cells. A.

Figure 1

: The mRNA expression of CXCL12 and CXCR4 in primary human trophoblast cells (labeled as PT) and JEG-3 cells was detected by RT-PCR (the upper panels). CXCR4 protein expression was detected by using western blotting (the lower panels). The GAPDH was used as a loading control. B: CXCL12 secretion in primary human trophoblast cells and JEG-3 cells was detected by ELISA. Human trophoblast cells were treated with 1 μM CsA in 1640 complete medium supplemented with 10% FBS for 48 h, and the culture medium was then collected and subjected to ELISA. Data are presented as mean ± SEM of three independent experiments. *P<0.05, compared to the control. Ctrl, control.