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. 2012 Jul 27;7(7):e38375. doi: 10.1371/journal.pone.0038375

Figure 2. CXCL12 promotes the phosphorylation of EGFR in primary human trophoblast cells and JEG-3 cells. A.

Figure 2

: Recombinant human CXCL12 (20 ng/ml) was used to treat the primary human trophoblast cells for 30, 60, 120 min, respectively. The levels of phosphorylated EGFR and total EGFR were detected by immunofluorescence analysis. Scale bar, 25 μm. B: Recombinant human CXCL12 (20 ng/ml) was used to treat JEG-3 cells for 30, 60, 120 min, respectively. The phosphorylated and total levels of EGFR were analyzed by western blotting. A typical blot (the lower panels) and densitometry analysis of the ratio of phosphorylated EGFR to total EGFR (the upper panels) are shown. Data are presented as mean ± SEM of three independent experiments. *P<0.05, compared to the control.