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(A) Semi-quantitative detection of biofilms formed by SE1457, WW06 in BM supplemented with 4% NaCl, 2% EtOH or 4% EtOH in 96-well polystyrene microtiter plates: The biofilms were stained with 2% crystal violet. (B) The crystal violet was dissolved in ethanol and the absorbance was determined at 570 nm. The mean value ± standard deviation for each strain is shown.
