Table 2. Primers used in this study.

Name	Primers	Applications
ICAP-S	5′-CTTTCAATTCTAAAATCTCCCCCTT-3′	icaADBC promoter
ICAP-AS	5′-TTTTTCACCTACCTTTCGTTAGTTA-3′	icaADBC promoter	EMSA
ICAA-S	5′-GAGGGAATCAAACAAGCA-3′	icaA fragment
ICAA-AS	5′-AGGCACTAACATCCAGCA-3′	icaA fragment
U-S	5′-CCGGAATTCTGGAACCGTATCGACAC-3′	arlS (upstream)
U-A	5′-CGCGGATCCGGTGGTATGATTCAGGTTG-3′	arlS (upstream)	Gene knockout
D-S	5′-AACTGCAGCGAATCACATACCCTACG-3′	arlS (downstream)
D-A	5′-CTAGCTAGCCTTGTATGTGGGGGGAAT-3′	arlS (downstream)
U-S’	5′-CGCTAAAGAAATACGTTGT-3′	arlS (upstream)
U-A’	5′-GCGCTAGGGACCTCTTTA-3′	arlS (upstream)
D-S’	5′-CTATTGTGAGTTATTAGTGG-3′	arlS (downstream)
D-A’	5′-AAGTGTCAAAGGTCTACTG-3′	arlS (downstream)	Mutant validation
erm-S	5′-CTATTGTGAGTTATTAGTGG-3′	ermB cassette
erm-A	5′-GCGCTAGGGACCTCTTTA-3′	ermB cassette
arlS-S'	5′-TCAACCTGAATCATACCACC-3′	arlS amplification
arlS-A'	5′-GATGCTTATTACGACGCTCAT-3′	arlS amplification
sarA-S	5′-TTCAAAAATCAATGACTGCT-3′	sarA amplification
sarA-AS	5′-TTCCTCTTCTTTATTCTCAC-3′	sarA amplification
sigB-S	5′-TCACCTGAACAAATTAACCAATG-3′	sigB amplification
sigB-AS	5′-CACCTATTAGACCAACCATACC-3′	sigB amplification
arlS-S	5′-ATTATTCAAGGTCATCTCAA-3′	arlS amplification
arlS-A	5′-ATCTCGCTATTTATGTCTAC-3′	arlS amplification
icaA-S	5′-TTATTGGTTGTATCAAGCGAAGTC-3′	icaA amplification	Real-time PCR
icaA-AS	5′-TCCTCAGTAATCATGTCAGTATCC-3′	icaA amplification
luxS-S	5′-GAGCAGATGATTATATTGT-3′	luxS amplification
luxS-AS	5′-TGTTAGATTCACTTGTTG-3′	luxS amplification
rsbU-S	5′-TCTCTTCATACAGTCCAT-3′	rsbU amplification
rsbU-AS	5′-ATAGGTTCAGGTATTCCA-3′	rsbU amplification
icaR-S	5′-GGAGCACTAGATAATTGAACAT-3′	icaR amplification
icaR-S	5′-CATTGACGGACTTTACCAG-3′	icaR amplification
gyrB-S	5′-TAGTATTGACGAGGCATTAGCA-3′	gyrB amplification
gyrB-AS	5′-TATCCGCCACCTCCGA-3′	gyrB amplification